Positive displacement pipettes
Here you will find out more about how you can use BRANDTECH positive displacement pipettes to efficiently dispense high-viscosity, low-viscosity or foaming media.
The Transferpettor pipette operates on the principle of positive displacement
In contrast with air displacement pipettes, the piston is in direct contact with the aspirated liquid. The tightly fitting piston always wipes the walls of the tips completely clean - literally to the last drop. Therefore, results are always perfectly reproducible. There is no need to discard tips after each pipetting operation, since residual wetting is negligible. However, in cases where no carry-over can be tolerated, for example with infectious or radioactive media, a different BRAND Transferpette® model is recommended, such as an air displacement pipette with a disposable tip for convenient operation.
Convenient pipetting of difficult media!
The Transferpettor is the ideal device for pipetting viscous media, such as highly concentrated protein solutions, oils, resins and fats, up to a viscosity of 50,000 mm2/s. High density media up to 13.6 g/cm3, such as glycerine, mercury, sulphuric acid, etc., can be dosed without recalibrating. Media with high vapor pressure up to 500 mbar, such as alcohols, ethers and hydrocarbons, can be easily and reliably dosed with high accuracy. Media which tend to foam, such as tenside solutions, are no problem for the Transferpettor.
Frequently Asked Questions (Positive displacement pipettes)
How can I adjust my Transferpettor?
An adjustment as known from the Transferpette® is not possible. However, when using the calibrating gauge from the maintenance kit it is possible to change the position of the piston rod of the Transferpettor micro (all volume ranges type fix; type adjustable volume up to 100µl included). Details on the procedure can be found in the operating manual in chapter "Changing the piston rod".
I cannot press the pipetting button of my Transferpettor macro completely down the bottom. The seal reaches the end of the tip before. The volume is too low about 1 ml. What is the reason for this?
To avoid this problem you have to press the pipetting button fully down when attaching the new tip. Is the problem not solved, the piston rod is loose. Therefore, in the next step you have to tighten the piston rod locking device. Details on this can be found in the operating manual in the chapter "Changing the piston rod" part "Attaching the new piston rod".
My Transferpettor macro has air bubbles in the aspirated liquid. What can I do?
Different reasons can be the cause. Please see the chapter "Troubleshooting" in the operating manual!
Can I order the Transferpettor DAkkS calibrated?
All our liquid handling instruments can be ordered DAkkS calibrated with certificate for a certain additional charge per piece. For ordering please add "DAkkS/...." as a prefix to the corresponding catalog number, e.g., DAkkS/7018 07 for a Transferpettor, 2.5-10µl, DAkkS calibrated. The current price list will provide more details on the additional charge for your requested instrument.
There is liquid above the seal of my Transferpettor macro. What can I do?
The reason therefore is a damaged tip or a damaged seal. The operating manual describes how to replace the tip/seal.
My Transferpettor micro has air bubbles in the aspirated liquid. What can I do?
Different reasons can be the cause. Please see the chapter "Troubleshooting" in the operating manual!
Do I have to test my Transferpettor completely after every replacement of tip?
After a replacement of a volume determining spare part the instrument should always be tested completely.
Does BRAND offer glass capillaries for the Transferpettor 100-500µl?
For the Transferpettor with a nominal volume from 500µl and onwards BRAND only offers PP-Caps and PE-Seals.
The pipetted volume of my Transferpettor micro is too large. What can I do?
The reason is the capillary that does not fit properly. Unscrew the collet, push the capillary completely to the stop and finger-tighten the collet again. Information is given in the manual in chapter "Changing the capillary" in part "To fit a new capillary".